Research Information System
6. International Molecular Biology and Biotechnology, Afyonkarahisar, Turkey, 22 - 25 December 2017, vol.1, no.1, pp.16
Trehalose is a multi-functional sugar for S. cerevisiae. Trehalose continuously recycled to keep steady state level in yeast cytoplasm. Neutral trehalase enzyme which degrades trehalose, is essential for the trehalose recycling. Neutral trehalase is encoded by NTH1 and NTH2 genes. It is known that transcription NTH1 is activated by Msn2/4, stress activated transcription factors, that involves in the regulation of large set of genes in S. cerevisiae in response to physiological stresses. To be involved in continuous recycling of trehalose, NTH1 gene is also transcribed at steady state levels even under normal growth conditions. It is known that promotor regions of transcriptionally active genes are cleared by chromatin modifying complexes to provide access to transcription initiation complex. In this study, we have analyzed if the chromatin modifying complexes involves in the transcription of NTH1 gene under normal and also under stress inducing growth conditions. We tested the effect of Snf2p, catalytic subunit of Swi/Snf complex, on the transcriptional regulation of NTH1. We have found that NTH1 gene expression in Δsnf2 mutants was 6-fold higher than wild type yeast strain. Under nitrogen starvation NTH1 gene expression increased 8-fold in wild type yeast strain but no change was observed in Δsnf2 mutants. Interestingly, NTH1 gene expression increased 4- fold in wild type yeast strain and 2- fold in Δsnf2 mutant strain after addition glutamin, rich nitrogen source. Our results indicated that Swi/Snf nucleosome mobilizing complex is essential for the transcriptional regulation of NTH1 gene.