Akademik Veri Yönetim Sistemi
JOURNAL OF FOOD SAFETY AND FOOD QUALITY-ARCHIV FUR LEBENSMITTELHYGIENE, cilt.76, sa.2, 2025 (SCI-Expanded)
Background: Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of paratuberculosis, also known as Johne's disease, in ruminants and is associated with Crohn's disease in humans. Due to its resistance to pasteurization, MAP can be transmitted through contaminated milk and milk products, posing a food safety risk.Methods: This study aimed to detect and assess the viability of MAP in retail pasteurized and raw tank cow milk in & Scedil;anl & imath;urfa, Turkey, using the propidium monoazide (PMA)-quantitative polymerase chain reaction (qPCR) method. A total of 130 milk samples (50 pasteurized and 80 raw tank cow milk) were collected from local shops and dairies. Samples were tested for the presence of MAP, and viable bacteria were further quantified using PMA-qPCR.Results: MAP was not detected in any of the pasteurized milk samples. One (1.42%) raw milk sample tested positive for MAP, but further PMA-qPCR analysis indicated that the bacteria were not viable.Conclusions: The PMA-qPCR method can effectively determine the viability of MAP in milk. Raw bulk milk was found to be at risk of MAP contamination; thus, it is recommended that raw milk be consumed with caution, ensuring proper hygiene and storage, and ideally, should not be consumed raw due to potential public health risks.