Determination of Foodborne Pathogens and Some Hospital Isolates Biofilm Formation Ability

Altuntaş S., Kaya B., Cınar A.

International Congress on Biological and Health Sciences, Afyonkarahisar, Turkey, 26 February 2021 - 28 May 2022, pp.117-128

  • Publication Type: Conference Paper / Full Text
  • City: Afyonkarahisar
  • Country: Turkey
  • Page Numbers: pp.117-128
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Biofilm-forming ability of pathogens is a key factor for the persistence in both food plants and

hospital equipments and to exhibit virulence factors. Due to their biofilm structure, they

develop a mechanism of resistance to antimicrobial agents, biocides, and heat. The aim of this

study was to determine the biofilm formation potential of foodborne pathogens and some

hospital isolates. For this purpose, 17 foodborne pathogens such as Bacillus cereus DSM

4312, Escherichia coli ATCC 35218, Escherichia coli O157 NCTC 12900, Escherichia coli

ATCC 25922, Enterococcus faecalis ATCC 29212, Enterococcus faecalis NCTC 8213,

Klebsiella pneumoniae ATCC 70063, Listeria monocytogenes ATCC 7644, Micrococcus

luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC

29213, Staphylococcus aureus ATCC 33592, Staphylococcus aureus ATCC 6538,

Staphylococcus epidermidis ATCC 12228, Salmonella Typhimurium ATCC 14028,

Salmonella enteritidis, Listeria monocytogenes (isolated from frozen food industry plant) and

3 hospital isolates such as Acinetobacter baumannii AYE, Klebsiella pneumoniae,

Staphylococcus aureus MRSA. The assessment of biofilm formation of foodborne pathogens

and hospital isolates was undertaken using a crystal violet assay on microtitre plates. The

results show that all the tested foodborne pathogens produced biofilms. Among these

foodborne pathogens, E. coli O157 NCTC 12900, E. coli ATCC 25922, both of E. faecalis, L

monocytogenes ATCC 7644, S. enteritidis and S. Typhimurium ATCC 14028 were weak

biofilm producers, while K. pneumoniae ATCC 70063 and S. aureus ATCC 6538 produced

moderate biofilms. The rest of test pathogens were classified as strong biofilm producers.

Additionally, S. aureus MRSA of the hospital isolate produced biofilm but K. pneumoniae

was not capable of producing biofilms. Findings from this study indicate that the foodborne

pathogens are an important biofilm producer. In conclusion by adding different strains to the

strains screened in this study, a database may be created for future biofilm studies.