Alterations in blood parameters, DNA damage, oxidative stress and antioxidant enzymes and immune-related genes expression in Nile tilapia (<i>Oreochromis niloticus</i>) exposed to glyphosate-based herbicide

ACAR Ü., Inanan B. E., Navruz F. Z., YILMAZ S.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, cilt.249, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 249
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.cbpc.2021.109147
  • Dergi Adı: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

In this study, effects of different concentrations (0, 5, 10, 20, 30, and 40 mg/L) of glyphosate-based herbicide (GBH) on Nile tilapia (Oreochromis nikticus) were investigated after a 14-day of exposure. After determination of LC50 value, effects of GBH on hematological and serum biochemical parameters in blood samples, DNA damage, lipid pemxidation and catalase activity in liver tissues, expression levels of antioxidant enzyme-related genes (SOD, CAT, GPx, and GST) and immune-related genes (TGF-beta, TGF-alpha and IL1-beta) were evaluated. The LC50 value has been found as 44.4 mg/L for GBH. GBH exposure at all concentrations caused alterations in blood parameters. GBH induced oxidative stress in liver and DNA damage in blood. Antioxidant enzyme-related genes were significantly up-regulated to suppress oxidative stress. On the other hand, the expression levels of immunerelated genes decreased in fish exposure to particularly >= 20 mg/L GBH.