Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3

Canbaz M. C. , Simsek C. S. , Sezginturk M. K.

ANALYTICA CHIMICA ACTA, cilt.814, ss.31-38, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 814
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.aca.2014.01.041
  • Sayfa Sayıları: ss.31-38


We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.