In this study, the knowledge levels of the teachers working at İSTEK Schools serving in the center and districts of Istanbul regarding the responsibilities required by the Occupational Health and Safety Law 6331 were evaluated by considering their different demographic characteristics. Face-to-face survey method technique was applied to the participants in obtaining the study data. SPSS 24.0 statistical package program was used for data entry and analysis. As a result of the cross-table analysis, it was determined that the majority of the teachers agreed with the opinion that Occupational Health and Safety culture should be at every stage of life and that trainings play an active role in reducing dangers and risks. It has been determined that teachers who have received Occupational Health and Safety training have more information about what to do as a result of an occupational accident than those who have not received training. It has been determined that male teachers' knowledge about OHS risks is higher than female teachers. In addition, it has been determined that the Occupational Health and Safety training activities carried out in educational institutions increase the knowledge level of teachers. According to the results of the research, it is foreseen that organizing organizations such as exhibitions, seminars and training programs related to Occupational Health and Safety in schools will contribute to the creation of safety culture awareness.