In the present study, growth performance, serum biochemical, hematological parameters and disease resistance against S. iniae in Mozambique tilapia were determined after 60-day feeding period with ethanol extract propolis supplemented diets. Growth performance increased (P < 0.05) in 2 and 4 g kg(-1.) propolis supplemented groups. There were no changes in hematological values between all treatment groups (P > 0.05). Total protein, values in serum found significantly higher in P-2 group (9.31 +/- 0.66 g dL(-1)) compared to the P-0 (7.49 +/- 1.66 g dL(-1)). The highest serum cholesterol value was obtained in P-4 (206.76 +/- 24.57 mg dL(-1)) group and found significantly different from P-0 (169.79 +/- 17.11 mg dL(-1)). The lactate dehydrogenase, lysozyme and myeloperoxidase activity significantly increased in P-1, P-2, P-4 and P-8 groups. The highest lysozyme and myeloperoxidase activity values were obtained in P-2 and P-4 groups (for lysozyme 255.44 +/- 3.41 mg L-1, 260.55 +/- 4.29 mg L-1 and for myeloperoxidase 145.01 +/- 3.82 U L-1 151.35 +/- 4.17 U L-1 respectively) and found significantly different from P-0 group. Propolis supplementation to the diets showed no differences on other serum biochemical parameters such as glucose, triglyceride, glutamic pyrivic transaminase and glutamic oxaloacetic transaminase. After challenge with S. iniae, fish fed with 2 and 4 g kg(-1) propolis showed significant survival rates (72.55% and 68.63%, respectively) compared with other groups. This study indicates that growth performance and non-specific immune response of Mozambique tilapia can be enhanced by dietary supplementation with propolis and that the optimal level would be 2 g kg(-1).