In this study, the nutritional composition of gonad samples of Paracentrotus lividus collected from three different stations in the Sea of Marmara (Dardanelles) and the Northern Aegean Sea, Bozcaada Island during the summer season of 2012 was investigated. As a purpose of the research, the effect of total carotenoids and free radical-scavenging activity assay of three different extracts (methanol, acetone and chloroform extracts) have been determined. Total carotenoids were found to range between 131 and 179 mu g/g of sea urchin. The current study shows that the highest inhibition was found as 99.1 (%) in methanolic extract sampled from Tuzburnu (the first station). Macro (Ca, Na, K and Mg) and micro (Cu, Fe, Mn and Zn) mineral composition of urchins was specified by ICP-OES. Results show that sea urchin was found to be rich in total carotenoids, antioxidant capacity, macro and micro minerals.