The wide range of applications of the plasma-based electron beam generator make it necessary to diagnose the device with a noninterfering method. The results of experimental and modeling studies of neutral helium and hydrogen beta spectral lines emitted from the double discharge pulsed electron beam generator are presented in this paper. Neutral helium lines emitted from the plasma in the pressure range 0.1-0.4torr are studied and compared with results of the collisional radiative model. The duration of the electron beam is shorter than 100 ns, and the peak current intensity is of order amperes. The full width at half maximum of the H spectral line is used for the determination of the plasma electron density, found as 3.16 x 10(21) m(-3) at 0.3 torr, and good agreement is obtained by comparing with the full computer simulation method.