The soil seedbank density is an approach to understand weed biology and to develop suitable integrated weed management tactics. Seedbank at cotton-wheat cropping system in the Cukurova Region of Turkey was studied. In total 30 fields were examined for the composition and density of the soil seedbank during three years. In this paper relation between weed flora and weed seedbank was compared for 5, 10 and 25 cm soil depths. There was a correlation of the seed number from different depths at all three years. The number of weed species was closer among species. But, the number of seeds and species in the seedbank were greatly varied among fields. Almost in all fields more weed species were detected above-ground than in the soil seedbank for all sampling depths. Portulaca oleracea or Amaranthus spp. were the most abundant species in the soil seedbank in all depths except in a few fields. The mean recruitment ratio from the seedbank varied. Sampling depth of 10 cm can be used for seedbank studies.