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4. International Congress on Applied Biological Sciences (ICABS), Eskişehir, Turkey, 3 - 05 May 2018, vol.1, no.1, pp.1, (Summary Text)
Trehalose is a non-reducing disaccharide that plays numerous important bio-functions in yeast. Neutral trehalase enzyme, encoded by NTH1 gene, responsible from the hydrolysis of intracellular stress accumulated trehalose in Saccharomyces cerevisiae. Transcription of NTH1 is activated by Msn2/4 which are stress activated transcription factors. For transcription to occur, DNA must be exposed to polymerase proteins and transcription factors by providing mobilization of nucleosomes through remodelers, in a process known as chromatin re-modeling. SWR1 chromatin remodeler, containing the catalytic subunit Swr1p, exchange H2A.Z-H2B dimers for canonical H2A-H2B dimers, which serves to specialize chromatin regions. In this study, we have analyzed if the SWR1 chromatin modifying complex involves in the transcription of NTH1 gene under nitrogen starvation conditions by using Δswr1 and wild type yeast strain. From the results, there was no difference between wild type and Δswr1 yeast strain before stress was given. In addition, we found that NTH1 transcription is 2-fold increased in Δswr1 yeast strain and 4-fold in wild type yeast strain after addition prolin. Our results indicated that SWR1 chromatin remodeling complex is not necessary in basal transcription of NTH1 gene but it is essential for the activation of NTH1 gene under nitrogen starvation.