Nano-hydroxyapatite incorporated quince seed mucilage bioscaffolds for osteogenic differentiation of human adipose-derived mesenchymal stem cells

Cetin Genc Ç., Yilmaz H. D., Karaca B., Kiran F., Arslan Y. E.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.195, pp.492-505, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 195
  • Publication Date: 2022
  • Doi Number: 10.1016/j.ijbiomac.2021.12.054
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.492-505
  • Keywords: Quince seed mucilage, Nano-hydroxyapatite, Bioscaffold, Osteoinductive, Non-load bearing bone grafts, Bone tissue engineering, BIOMATERIAL SCAFFOLDS, TISSUE, ADHESION, CHITOSAN, COLLAGEN, OSTEOPONTIN, DEGRADATION, HYDROGELS
  • Çanakkale Onsekiz Mart University Affiliated: Yes


In this study, the therapeutic hydrocolloid quince seed mucilage (QSM) from Cydonia oblonga Miller fruit is enriched with needle-like nano-hydroxyapatite (nHAp) crystals to fabricate a novel biomimetic osteogenic bioscaffold. The molecular weight (M-w) of water-based extracted QSM was measured with GPC (8.67 x 10(5) g/ mol), and the composite blend was prepared at a ratio of 1:1 (w/w) QSM(aq) and nHAp. The porous bioscaffolds were manufactured by the freeze-drying method, and evaluated in-depth with advanced analyses. The XRD, ATR-FTIR, SEM-EDX, and elemental mapping analyses revealed a uniform coated semi-crystalline structure with no covalent bindings between QSM and nHAp. Moreover, due to the hydrocolloid backbone, a supreme swelling ratio (w/w, 6523 +/- 190%) with suitable pore size (208.12 +/- 99.22 mu m) for osteogenic development was obtained. Further, the cytocompatible bioscaffolds were evaluated for osteogenic differentiation in vitro using human adipose-derived mesenchymal stem cells (hAMSCs). The immuno/histochemical (I/HC) staining revealed that the cells with the spherical morphology invaded the pores of the prepared bioscaffolds. Also, relatively early up-regulated osteogenic markers were observed by the qRT-PCR analyses. Overall, it is believed that the QSM-nHAp bioscaffolds might be favorable in non-load bearing applications, especially in the cranio-maxillofacial region, due to their regenerative, bendable, and durable features.