The Effects of Branched Chain Amino Acid Supplement on Kidney Tissue of Exercising Rats

Aydeğer C., Eroğlu H. A.

REVISTA DE NEFROLOGIA, DIALISIS Y TRANSPLANTE, vol.42, no.2, pp.124-133, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 42 Issue: 2
  • Publication Date: 2022
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, Directory of Open Access Journals, DIALNET
  • Page Numbers: pp.124-133
  • Çanakkale Onsekiz Mart University Affiliated: Yes


 Introduction: One of the supplements used in exercise programs are branched-chain amino acids (BCAAs), which are preferred because of their effect on the regeneration of muscle protein synthesis. However, due to their properties, BCAAs increase their amount in the blood in a short time. In this case the result may increase the workload of the kidneys. Based on the information, this study investigated the effects of resistance exercise and BCAA supplements on kidney tissue. Methods: A total of 24 Wistar Albino male rats were equally divided into 4 groups: Control, BCAA, Exercise and Exercise + BCAA. In the six-week study, resistance swimming exercise was applied to the exercise groups. BCAA supplementation was given to BCAA groups at 2.5 mg/kg doses before exercise. At the end of the study, histological, immunochemical and RT-PCR analyzes were performed. Results: As a result of the findings, it was found that the use of BCAA supplements together with exercise caused tubular necrosis (p=0.002). There was a significant increase in caspase 3 IHC staining findings in BCAA and Exercise + BCAA groups compared to the control group (p=0.011; p=0.02). In addition, KIM- 1 expression levels were higher in the Exercise group than in all other groups (p=0.004; p = 0.003; p=0.008). Conclusion: As a result, BCAA consumption with resistance exercise caused damage to kidney tissue.