This study aimed to determine aphid species, and to contribute new species and hosts, in Canakkale and Balikesir Provinces in the South Marmara Region of Turkey. Samples were taken from different host plants between March 2017 and November 2018. In total 74 aphid species were identified, including three subspecies from 34 genera belong to five subfamilies (Aphidinae, Calaphidinae, Chaitophorinae, Eriosomatinae and Lachninae) of the family Aphididae. Among these, Dysaphis radicola meridialis Shaposhnikov, 1964 collected from the roots of Rumex sp. (Polygonaceae) is a new subspecies record for the aphid fauna of Turkey from Canakkale. The new host records for Turkey are Ajuga orientalis L. (Lamiaceae) on Aulacorthum (Aulacorthum) solani (Kaltenbach, 1843), Cynoglossum creticum Mill. (Boraginaceae) on Acyrthosiphon (Acyrthosiphon) malvae (Mosley, 1841) and Tragopogon porrifolius L. (Asteraceae) on Trama (Neotrama) caudata Del Guercio, 1909. Also, body measurements, diagnostic features, illustrations, distribution and biology are given for the new aphid records. With this contribution, the number of aphids in the fauna of Turkey has reached to 541 species and 14 subspecies.