In this study, some biochemical parameters (glucose, urea, calcium, triglyceride, cholesterol, creatinine, uric acid, total protein, iron, albumin, phosphorus, H.D.L., magnesium) hemoglobin and hematocrit depending on the pre-reproductive and reproductive periods and sex were determined in a total of 20 Testudo graeca specimens in the canakkale (Turkey). For the biochemical parameters, 0,5 ml of blood was collected from the scale in the lateral ligament of the neck with a 5-ml aspirating syringe. The blood was transferred into the biochemical tubes and centrifuged; the plasma part was separated and was measured by spectrophotometer. In conclusion, plasma biochemistry parameters were determined on sex-related pre-reproduction and reproduction periods in T. graeca. When comparing the pre-reproductive and the reproductive period in terms of glucose, uric acid and iron values, statistical differences were found in the females. It was determined that cholesterol values of males were significantly different between periods. In the females, glucose value was found high in the pre-reproductive period and uric acid and iron values were high in the reproductive period. Cholesterol values of males were found high in the pre-reproductive period. Iron levels in the pre-reproductive period were significantly different between males and females.