Cloning and characterization of genes concerning Na+/H+ antiport in maize

Zörb C., Sümer A., Schubert S.

Botanikertagung 2000, Jena, Germany, 17 - 22 September 2000, pp.58

  • Publication Type: Conference Paper / Summary Text
  • City: Jena
  • Country: Germany
  • Page Numbers: pp.58
  • Çanakkale Onsekiz Mart University Affiliated: No


Salinity stress is one of the most serious factors limiting the productivity of agricultural crops. A key of understanding the physiology of salt tolerance in plants is to identify genes and proteins of the pathways for Na+-transport across the plant cell membranes. We have developed an inbred line which shows efficient Na+-exclusion (Schubert, 1999). Two different Na+-sensitive maize genotypes were cultivated in nutrient solution in a growth chamber. Plants were harvested after one week of salt treatment (100 mM NaCl) and compared with non-treated plants. Total m-RNAfron root material of the different genotypes with and without salt treatment were isolated and transcribed into cDNA. Primers were designed according to Na+/H+ antiporter from O, sativa (OsNHX1, Acc. NoAB021878) and S. pombe (Sod 2, Acc. No Z11736). Using the PCR technique two different fragments of the putative Na+/H+ antiporterwere isolated from root material of Na+ excluding genotypes. To monitor the protein expression pattern of maize genotypes 2-D gelelectrophoresis were carried out. In comparison with the efficient and poorly excluding genotypes we found some differentially expressed protein spots especially in the range of the isoelectric point and the molecular weight of the putative plant Na+/H+ antiporter.

Schubert 1999; In: W. Merbach, L. Wittenmayer, J. augustin, Hrsg., Stoffumsatz im wurzelnahen Raum, Stuttgart-Leipzig, 74-79