The role of SWR1 and SWI/SNF complex on TPS1 gene expression in Saccharomyces cerevisiae

Turgut Genç T., Akıncı N.

2. International Rating Academy Congress on HOPE, Çanakkale, Turkey, 19 - 21 April 2018, vol.1, no.1, pp.1

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Çanakkale
  • Country: Turkey
  • Page Numbers: pp.1
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Trehalose has several important physiological roles in Saccharomyces cerevisiae, such as stress protector to environmental stress conditions. TPS1 encoded trehalose-6-phosphate synthetase enzyme is one of the catalytic subunit of TPS complex. TPS1 gene includes STRE sequences for binding of Msn2/4 transcription factors during stress response process. Chromatin remodeling complexes allow DNA to be transcriptionally accessible for binding of transcription activators and repressors. The SWR1 chromatin remodelling complex replaces the canonical histone H2A with the variant H2A.Z at specific chromatin regions. SWI/SNF complex disrupts the nucleosome structure, mobilizes histone octamers. In this study we investigated the role of SWR1 and SWI/SNF chromatin modifying complexes in transcription of TPS1 gene. Δswr1 and Δsnf2 mutant yeasts and their wild type yeast strain were used for determining the promoter induction under nitrogen starvation. We were found that there was no difference in TPS1 gene expression of Δsnf2 mutant yeast strain with respect to wild type. However in Δswr1 mutant yeast strain, TPS1 transcription was 2-fold lower than wild type both in normal and sstarvation conditions. Our results indicated that SWI/SNF chromatin remodeling complex has no effect on TPS1 gene regulation, but SWR1 chromatin remodeling complex is essential for both basal and activated transcription.