This study was carried out to investigate and compare 40 wheat bread genotypes with different origins for their agronomic traits, grain yield, flour quality traits, and gliadin band variations. The field trial was conducted in 20082009 and 2009-2010 growing seasons at the Dardanos Research and Application Center of canakkaie Onsekiz Mart University, in canakkaie, Turkey. A new statistical method, calibrated biplot analysis, was used to compare the genotypes for the investigated traits, based on their gliadin band variation. Gliadin band analysis resulted in 7 different genotype groups, and significant variations were detected within each group. The genotypes originated in proximate or similar regions were found to be genetically close as suggested by the gliadin band analysis. Biplot analysis detected that some genotypes had similarities in terms of agronomic and quality traits within their respective gliadin band groups, whereas it was not quite possible to make a clear distinction for all of the genotypes. Overall results suggested that Sagittario, one of the prevalent varieties of the region, could be recommended to growers as a high yielding and high quality cultivar; while the other widely grown cultivars (i.e., Gonen-98 and Kasifbey-95) were inferior to some other genotypes (Selimiye, Zajecarska-75, Guadelupe) in terms of yield and quality, under the conditions of experimental years.