Akademik Veri Yönetim Sistemi
3rd International Symposium on Biodiversity Research, Erzurum, Türkiye, 20 - 22 Ekim 2021, ss.238-247
Paeonia species are known peony and used as a folk medicine in the treatment of many diseases. These species, which have herbaceous and woody forms, are rooted plants with a tuberous base and fleshy roots. Paeonia mascula subsp. bodurii subspecies is an endemic plant and distributed in Çanakkale, Turkey. The conservation status of this species has been declared as Endangered (EN) according to the red data book of Turkish Plants. It takes 2 years for the seeds to germinate in nature, as their seeds have dormancy one after the other. For this reason, plant tissue culture techniques are used for their production. The aim of this study, establish to in vitro micropropagation of Paeonia mascula subsp. bodurii. Shoot tip explants were cultured on ½ MS medium supplemented with 1 mg/L BAP, 1 mg/L GA3, 1 g/L PVP, 3% (w/v) sucrose and 0.7% (w/v) agar for shoot induction. All cultures were maintained at 25±2°C under the 16/8 h photoperiod with a light intensity of 72 μmol m-2s-1. The shoots were cultured on ½ MS medium containing 1 mg/L IAA and 0.3 g/L AC in darkness at +4°C for different period (10, 15, and 20 days) for shoot development and tuberous fleshy storage root induction. Each stage of micropropagation for P. mascula had been optimized under optimum conditions, shoot and tuberous fleshy storage root induction treatments were compared. Shoot induction were achieved with SI medium. Our result showed that culturing the shoots on ½ MS medium containing 1 mg/L IAA and 0.3 g/L AC in darkness at +4°C for 15 days was effective for shoot development but these conditions were insufficient for tuberous fleshy storage root induction.