Chitosan-Coated Magnetic Nanoparticles for the Efficient Capture of Salmonella Typhimurium

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Eren D., Eser E.

7th Eurasia Biochemical Approaches & Technologies (EBAT), Antalya, Türkiye, 6 - 09 Kasım 2025, ss.9, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.9
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Glycan-coated magnetic nanoparticles (gMNPs) have emerged as a novel and cost-effective approach for the rapid concentration and recovery of foodborne pathogens. Unlike traditional immunomagnetic separation (IMS), which relies on antibody-based magnetic beads that are expensive and sensitive to storage conditions, gMNPs offer a more stable, economical, and adaptable alternative. This approach leverages the natural affinity between glycans and bacteria, enabling their capture directly from complex food matrices (1).

In this study, chitosan-coated Fe3O4 magnetic nanoparticles (CS-MNPs) were synthesized using two methods: a one-pot hydrothermal synthesis, in which chitosan was incorporated during nanoparticle formation, and a post-synthesis surface coating technique. The nanoparticles were characterized by confirming successful chitosan functionalization and favorable magnetic properties. The performance of the CS-MNPs was evaluated for the recovery of Salmonella Typhimurium ATCC 14028 from pure cultures and ultra-high temperature (UHT) milk samples artificially contaminated with bacterial loads ranging from 10¹ to 10⁷ CFU/mL. Both synthesis approaches yielded functional CS-MNPs, achieving recovery rates greater than 50%, depending on the synthesis method and initial inoculum level.

This study highlights the potential of CS-MNPs as a practical and cost-efficient alternative to IMS for pathogen recovery in food safety applications. While glycan-coated particles may exhibit lower intrinsic specificity compared to antibody-based methods, specificity can be enhanced through secondary detection strategies, such as biosensor integration. Additionally, targeted modifications-such as functionalizing gMNPs with specific molecular probes -can further improve selectivity toward particular pathogens. To fully establish the versatility and application range of this approach, future studies should explore its performance across different bacterial species and food matrices.

Acknowledgments:

This study was supported by the Scientific Research Projects Coordination Unit of Canakkale Onsekiz Mart University (Project No: FYL-2025-5075) and by TUBİTAK (The Scientific and Technological Research Council of Türkiye) under project number 124Z510.