A feeding experiment was conducted to study the response of rainbow trout juveniles fed different levels of lupin meal in diets for rainbow trout juveniles. Very limited information is available on the relationship between dietary lupin meal in rainbow trout health status. Therefore, this study aimed to determine the effects of lupin meal inclusion levels (0%, 15%, 30%, 45%, and 60%) on growth performance and health status of rainbow trout juveniles. The experimental diets (LM0, LM15, LM30, LM45, and LM60) were formulated iso-nitrogenous (41% crude protein) and iso-calorific (18% crude lipid). The fish were fed twice a day. As a result, the best growth performance was observed in fish fed with LM15 and LM30 diets. No significant differences were detected among experimental groups in terms of body compositions. The haematological values showed significantly (p<0.05) lower heamatocrit and mean cellular volume (MCV) in the group of LM60 compared with the other groups. For the other haematological parameters such as haemoglobin, red blood cell and mean cellular haemoglobin studied in the present study no significant differences were observed (p<0.05). The lupin meal included groups showed significant reduction in total protein (TPROT), triglyceride (TROG), cholesterol (CHOL), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) compared with the control group (p<0.05). The inclusion of lupin meal did not cause any changes in glucose (GLU), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) between the treatment groups (p<0.05). In conclusion, lupin meal might be used in rainbow trout diets up to 30% without any malnutrition effect on growth performance, haemotological and serum biochemical parameters.