Akademik Veri Yönetim Sistemi
UNION OF THRACE UNIVERSITIES VI. INTERNATIONAL HEALTH SCIENCES CONGRESS, Edirne, Türkiye, 30 Kasım - 01 Aralık 2023, cilt.1, sa.1, ss.22
Aim: Pseudomonas aeruginosa is an important nosocomial pathogen that can be isolated from environmental sources, primarily freshwater and soil, and rarely from skin microbiota. Resistance to antibiotics and even multidrug (MDR) is increasing worldwide. This species can acquire natural resistance to many classes of drugs and all related treatments. Besides antimicrobial resistance (AMR), biofilm formation is another important factor that increases the virulence of bacteria. AMR particularly complicates the management of pathogenic bacteria. One of the potential options for new antimicrobial agents against increasing AMR is plants. Essential oils (EO) from plants have been used in folk medicine to treat diseases for many years. Therefore, this study investigated the in vitro antibacterial activity of 19 commercial essential oils against P. aeruginosa.
Methods: Antibacterial activity was evaluated against P. aeruginosa (Schroeter) Migula ATCC 10145 and ATCC 27853 by agar well diffusion method. The analysis was carried out in three parallels. Results are given as mean (M) ± standard deviation (SD).
Results: The antibacterial activity of 11 (Anemone apennina EO, Adiyaman mint oil, moss fixed oil, Citrus sinensis fixed oil, Allium sativum oil, Lavandula angustifolia EO, L. stoechas EO, Jasminum sp. EO and Viola odorata EO from 3 different companies) of the 18 oils tested could not be determined. Toros mint oil, Cinnamomum sp. and Mentha piperita EO were 7.38±0.52, 11.00±0.76, and 13.63±1.60 mm, respectively, against 10145, and it gave inhibition zones of 13.63±1.06, 12.50±1.07, and 16.38±1.30 mm against 27853. While the antibacterial activity of one of the Jasminum sp. oils belonging to two companies could not be determined, the J. officinale flower oil containing propylene glycol had an antibacterial activity of 15.50±0.93 mm against 14053 and 7.13±0.35 mm against 27853. It was determined that Citrus limon seed and C. limon EO gave an inhibition zone of 15.56±0.98 and 22.25±1.67 mm, respectively, against 10145 and 15.00±0.76 and 23.88±0.99 mm, against 27853. When the levofloxacin (5μg) antibiotic disk was used as a positive control in the study, it gave 39.89±1.60 and 40.52±0.82 mm inhibition zones against 10145 and 27853, respectively, Thymus sp. EO gave 38.50±2.73 and 44.75±1.67 inhibition zones, respectively.
Conclusions: The effectiveness of Thymus sp. EO against P. aeruginosa is important and indicates that it can be used for treatment. In addition, it is observed that the antibacterial activity is different due to the same oil being produced by different companies. For this reason, the importance of determining the chemical composition of oils in studies on antimicrobial activity has been emphasised again.