Changes in β3 receptor levels in diabetes mellitus and infravesical obstruction-induced cystopathy in rat bladders

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Tezcan S., Koşan T. M., Güven Bağla A., Sancak E. B., Elmas S.

ANNALS OF CLINICAL AND ANALYTICAL MEDICINE, cilt.17, sa.5, ss.407-412, 2026 (ESCI)

Özet

Abstract

AimThis study aims to immunohistochemically determine potential changes in the number of β3 receptors caused by diabetes and bladder outlet obstruction (BOO), an ailment whose frequency increases with age.
MethodsFour distinct groups, each comprising 12 rats, were established: a control group, a diabetic group, an obstructive group, and a diabetic obstructive group. To induce moderate obstruction, a 5 Fr catheter was utilized. For moderate diabetes induction, a dose of 50 mg/kg STZ was intraperitoneally administered to the rats. Over 8 weeks, the rats’ blood sugar and body weight levels were closely monitored. After euthanasia, the rats’ bladder tissues were extracted for subsequent histological and immunohistochemical assessments. Statistical analyses were conducted to compare the results, with significance attributed to p-values < 0.05.
ResultsThe histopathological examination revealed notable variances among the groups concerning urothelial hyperplasia and inflammation. Moreover, a discernible increase in intensity of β3 receptor staining was noted in the obstructive group when compared to the control group, whereas a decrease was observed in both the diabetic and diabetic obstructive groups.
ConclusionAlterations in receptor count may not necessarily correspond to equivalent changes in receptor functionality. Consequently, a comprehensive evaluation of receptor function necessitates diverse in vivo and in vitro investigations.