Nrg1 is suppressor of NTH1 gene in Saccharomyces cerevisiae


Turgut Genç T. , Doğan G.

7. International Molecular Biology and Biotechnology Congress (MOLBIOTECH), Konya, Turkey, 25 - 27 April 2018, vol.1, no.1, pp.1

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.1

Abstract

The amount of intracellular trehalose increases in response to environmental stress in yeast Saccharomyces cerevisiae. Whenever the stress isterminated, the accumulated trehalose was degraded into glucose rapidly. Synthesis of the trehalose was fulfilled by the TPS enzyme complex, whereas the degradation of trehalose was done by the neutral trehalase enzyme. By the action of these two enzymes, trehalose level in the cell is maintained at a certain level. Under different stress conditions, the transcription of NTH1 gene is activated and Stress Response Elements (STRE) are required for this activation. Nrg1 protein can bind promoters including STRE and PDS elements. Because of the presence of three possible Nrg1 repressor binding sites on NTH1 promoter, NTH1 gene may be regulated by Nrg1 repressor. In order to test this, Δnrg1 mutant yeast and its isogenic wild-type yeast strain were used to analyze NTH1 gene induction under nitrogen starvation conditions.Nth1 transcription was 7 fold higher than that of wild type at normal growth conditions, and was not changed during nıtrogen stravation. These results showed that NTH1 gene expression is constitutive in the absence of Nrg1 repressor protein, and so Nrg1 is a suppressor of NTH1 gene.