ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF CHERRY LAUREL LEAF AND FRUIT EXTRACTS


Özçelik E., Uysal Pala Ç.

ISISTANBUL INTERNATIONALMODERN SCIENTIFIC RESEARCH CONGRESS-III, İstanbul, Türkiye, 6 - 08 Mayıs 2022, ss.1131-1138

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1131-1138
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Cherry laurel (Prunus laurocerasus L.) was grown naturally on the Black Sea coast of Turkey. It had green leaves in all seasons and was used for landscaping purposes. Its dark green leaves and red-purple fruits, also known as "Taflan", are used traditionally for food and health purposes. Within the scope of this study, it was aimed to determine the antioxidant properties of leaf and fruit extracts by total phenol, DPPH (2,2-diphenyl-1-picryl-hydrazy hydrate), and CUPRAC (cupric ion reducing antioxidant capacity) methods and to investigate the antimicrobial effect on important pathogenic microorganisms including Escherichia coli(ATCC 25922), Salmonella Typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 9144), Methicillin Resistant Staphylococcus aureus, Listeria innocua (ATCC 33090), Candida albicans (ATCC 10231) and Bacillus cereus (ATCC 10876) by using the disk diffusion method. In terms of total phenolics, the fruit extract gave the highest value with 76.33±1.60 mg GAE/g. The fruit extract was followed by the leaf-alcohol extract with a concentration value of 56.93 ±3.67 mg GAE/g. When the data obtained from both CUPRAC and DPPH antioxidant methods were evaluated, it was determined that the leaf-alcohol extracts were the strongest in terms of antioxidant capacity (P<0.05). In parallel with this result, when the inhibition zones of fruit and leaf (water and alcohol) extract on pathogens in mm were compared, it was found that leaf-alcohol extracts showed the highest antimicrobial effect. With the leaf-alcohol extract, the inhibition effect was determined on all microorganisms studied except E. coli. On the other hand, an inhibition effect was observed with fruit extract only on MRSA, S. aureus, L. innocua, and B. cereus. In conclusion, cherry laurel leaf-alcohol and fruit extracts have the potential to be natural agents with antioxidant and antimicrobial properties.