Fifth International Symposium on Medicinal Plants & Materials. MPM 2024 09-12 May 2024, Skanès Monastir, Tunisia, Monastir, Tunisia, 9 - 12 May 2024, pp.65
This research aimed to chemical composition of the essential oil (EO) extracted from
the peels of Citrus limon and to evaluate its antioxidant, antibacterial and insecticidal activities
against rice weevil adults. Essential oil is obtained by steam distillation with a yield of 0.576 ±
0.01% and analyzed by gas chromatography coupled to mass spectrometry (GC/MS). The
antioxidant activity of C. limon essential oil was investigated using DPPH, FRAP, and chelating
activity on ferrous ions methods. This essential oil exhibited a remarkable antiradical and
reducing power against DPPH, FRAP and chelating activity. The in vitro antibacterial tests
using the agar well diffusion method were carried out on four bacteria, such as Staphylococcus
aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli. The essential oil
demonstrated a considerable antibacterial activity against all bacterial strains. Insecticidal
assessments were carried out against storage food pest rice weevil. Two insecticidal activities
of the essential oil were performed using two different methods that contact toxicity and
repellent tests. The essential oil showed repellent and insecticidal effects against rice weevil.