Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing

Elbeaino T., Abou Kubaa R., Tuzlali H. T., Digiaro M.

ARCHIVES OF VIROLOGY, vol.161, no.7, pp.2039-2042, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 161 Issue: 7
  • Publication Date: 2016
  • Doi Number: 10.1007/s00705-016-2860-5
  • Journal Name: ARCHIVES OF VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2039-2042
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylogenetic and sequence analyses confirmed that PiCV1 is a new member of the genus Deltapartitivirus, family Partiviridae. From the same plant, NSG also permitted assembly of the complete genome sequence of eggplant mottled dwarf virus (EMDV), which shared 86 % to 98 % nucleotide sequence identity with complete and partial sequences (ca 6750 nt) of other known EMDV isolates with sequences available in the GenBank database.