Biochemical Responses of Two Bacteria Isolated from Çanakkale Coastal Zone on Barley Under Salt Stress

Creative Commons License

Teker Yıldız M., Acar O.

5th Eurasia Biochemical Approaches & Technologies Congress (EBAT ), Antalya, Türkiye, 2 Kasım - 05 Aralık 2023, cilt.1, sa.1, ss.136

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 1
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.136
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Salt stress is one of the major abiotic stresses that threaten the environment by negatively affecting sustainable agricultural productivity all over the world. However, plant growth promoting rhizobacteria (PGPR) are a sustainable environmentally friendly product that is highly effective in increasing plant resistance to environmental stresses.1 Within the scope of the study, the effects of Micrococcus luteus (ML) and Bacillus nealsonii (BN) isolated from the Çanakkale coastal region of Turkey and diagnosed with 16s rRNA inoculating on sea barley grass (Hordeum marinum subsp. marinum) and two cultivated barley varieties (Hordeum vulgare L. salt-tolerant cv. Ocak, salt-sensitive cv. İnce-04) under salt stress (0, 100, 300 mM NaCI). We focused on biochemical parameters (chlorophyll content, total protein content, hydrogen peroxide content (H2O2, spectrophotometric and histochemical staining), lipid peroxidation (TBARS), cell membrane permeability (ES)) and some antioxidant enzyme activities (peroxidase (POX), catalase (CAT)). Our results showed that chlorophyll content decreased, while TBARS, H2O2 content, and ES improved with increasing salt stress in İnce-04 variety. Besides H. marinum subsp. marinum was less affected by salt stress and were more tolerant salinity compared to other barley varieties. In addition, ML and BN inoculation eliminated negative impacts of salt stress with increased chlorophyll content, CAT, and POX activities in salt-sensitive İnce04. As a result, it reduced the negative effects of salt stress by reducing the production of reactive oxygen species (ROS) after PGPR inoculation. These results also show that ML and BN inoculation into barley can be used as biofertilizer under salt stress conditions.