Research Information System
5th Eurasia Biochemical Approaches & Technologies Congress (EBAT ), Antalya, Turkey, 2 November - 05 December 2023, vol.1, no.1, pp.136, (Summary Text)
Salt stress is one of the major abiotic stresses that threaten the environment by negatively
affecting sustainable agricultural productivity all over the world. However, plant growth promoting
rhizobacteria (PGPR) are a sustainable environmentally friendly product that is highly effective in
increasing plant resistance to environmental stresses.1 Within the scope of the study, the effects of
Micrococcus luteus (ML) and Bacillus nealsonii (BN) isolated from the Çanakkale coastal region of
Turkey and diagnosed with 16s rRNA inoculating on sea barley grass (Hordeum marinum subsp.
marinum) and two cultivated barley varieties (Hordeum vulgare L. salt-tolerant cv. Ocak, salt-sensitive
cv. İnce-04) under salt stress (0, 100, 300 mM NaCI). We focused on biochemical parameters
(chlorophyll content, total protein content, hydrogen peroxide content (H2O2, spectrophotometric and
histochemical staining), lipid peroxidation (TBARS), cell membrane permeability (ES)) and some
antioxidant enzyme activities (peroxidase (POX), catalase (CAT)). Our results showed that chlorophyll
content decreased, while TBARS, H2O2 content, and ES improved with increasing salt stress in İnce-04
variety. Besides H. marinum subsp. marinum was less affected by salt stress and were more tolerant
salinity compared to other barley varieties. In addition, ML and BN inoculation eliminated negative
impacts of salt stress with increased chlorophyll content, CAT, and POX activities in salt-sensitive İnce04. As a result, it reduced the negative effects of salt stress by reducing the production of reactive
oxygen species (ROS) after PGPR inoculation. These results also show that ML and BN inoculation into
barley can be used as biofertilizer under salt stress conditions.