Sequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativa L.)

Cil A., TİRYAKİ İ.

PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION, cilt.14, sa.3, ss.183-191, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1017/s1479262115000210
  • Dergi Adı: PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.183-191
  • Anahtar Kelimeler: DNA content, genetic relationship, genome size, inter-simple sequence repeat, polymorphism information content, sequence-related amplified polymorphism, Vicia sativa, GENOME SIZE VARIATION, INTRASPECIFIC VARIATION, MOLECULAR MARKERS, EVOLUTION, FABACEAE, SRAP, RAPD
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Genetic diversity of 30 common vetch (Vicia sativa L.) lines and cultivars obtained from various resources or collected from natural flora of Turkey was evaluated by using 55 sequence-related amplified polymorphism (SRAP) and five inter-simple sequence repeat (ISSR) primer sets, and their nuclear DNA contents were determined by flow cytometer. A total of 188 polymorphic loci were detected, with an average of 3.62 loci per primer. The percentage of polymorphic loci was 82.1%. The polymorphism information content values ranged from 0.12 to 0.96, with an average of 0.63. The genetic distance coefficients were in the range of 0.112-0.627. Cluster analysis revealed that the 27 lines and three cultivars could be divided into two main groups. No polyploidy was detected within any vetch lines tested while significant (P<0.0001) nuclear DNA content differences were determined. The present study revealed that fast and accurate fingerprinting analysis could be done using SRAP and ISSR markers, which indicated existing significant variation among common vetch lines and cultivars.