Research Information System
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, vol.14, no.1, pp.41-46, 2015 (SCI-Expanded)
Purpose: To investigate the antioxidant and anti-melanogenesis activities of Durio kutejensis [Bombacaceae (Hassk.) Becc] fruit extract. Methods: The fruit flesh of Durio kutejensis was extracted successively with n-hexane, ethyl acetate/EtOAc, and ethanol/EtOH at room temperature repeatedly. The extracts were concentrated in vacuo to yield their residues. Antioxidant properties were analyzed by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS, superoxide dismutase (SOD)-like activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) while anti-melanogensis activity was evaluated by tyrosinase enzyme activity and B16 melanoma cell assays (melanin inhibition and cytotoxicity). Results: The extract (200 mu g/mL) showed melanin inhibition by inhibiting melanin formation in B16 melanoma cell by 47% without cytotoxicity but did not inhibit tyrosinase enzyme activity. The extract (1 - 1000 mu g/mL) also exhibited some level of antioxidant activity including ORAC (0.04 +/- 0.00 mu mol TE/mg at 950 mu g/mL), ABTS (1.0 +/- 0.2% at 100.8 mu g/mL), SOD (IC50, 76.00 +/- 14.6 mu g/mL, and DPPH (21.5 +/- 0.7% at 97.39 +/- g/mL extract concentration). Conclusion: The fruit extract of Durio kutejensis has antioxidant properties with a potential for treating hyperpigmentation and for use as a skin-lightening agent.