Weissella cibaria EIR/P2-derived exopolysaccharide: A novel alternative to conventional biomaterials targeting periodontal regeneration

Kibar H., ARSLAN Y. E., Ceylan A., Karaca B., Haliscelik O., Kiran F.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.165, pp.2900-2908, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 165
  • Publication Date: 2020
  • Doi Number: 10.1016/j.ijbiomac.2020.10.106
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.2900-2908
  • Keywords: Biofilm, Dextran, Human periodontal ligament fibroblast cells, Weissella cibaria
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Healing and regeneration of periodontium are considered as a complex physiological process. Therefore, treatments need to be addressed with highly effective components modulating the multiple pathways. In this study, exopolysaccharide (EPS) produced by Weissella cibaria EIR/P2, was partially purified from the culture supernatant and subjected to characterization within the aim of evaluating its potential for periodontal regeneration. High-Performance Liquid Chromatography analysis revealed a single-peak corresponding to the glucose which identified the EPS as dextran. Fourier transform-infrared spectra were also displayed characteristic peaks for polysaccharides. According to the results of gel permeation/size exclusion-chromatography, the molecular mass was determined to be 8 x 10(6) Da. To clarify its anti-bacterial activity on Streptococcus mutans, effects on viability and biofilm formation was evaluated. At 50 mg/mL, dextran exhibited a bactericidal effect with 70% inhibition on biofilm formation. Besides, dose-dependent antioxidant effects were also detected. The efficacy of dextran in enhancing the viability of human periodontal ligament fibroblast cells (hPDLFCs) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, and an increase was observed in the viability of hPDLFCs. In conclusion, dextran derived from W. cibaria can be potentially used as a multi-functional bioactive polymer in the design of new therapeutic strategies to promote healing and regeneration of periodontium. (C) 2020 Elsevier B.V. All rights reserved.