Dataset on the differentiation of THP-1 monocytes to LPS inducible adherent macrophages and their capacity for NO/iNOS signaling


Ozleyen A., YILMAZ Y. B. , TÜMER T.

DATA IN BRIEF, vol.35, 2021 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35
  • Publication Date: 2021
  • Doi Number: 10.1016/j.dib.2021.106786
  • Journal Name: DATA IN BRIEF
  • Journal Indexes: Emerging Sources Citation Index, Scopus, BIOSIS, Directory of Open Access Journals
  • Keywords: Nitric Oxide, iNOS, Inflammation, THP-1 monocytic cells, PMA differentiation, NITRIC-OXIDE SYNTHASE, FACTOR-KAPPA-B, CELL-LINE, MODULATION, ACTIVATION, GAMMA

Abstract

When THP-1 cells are differentiated into adherent macrophage-like cells, they respond to inflammatory stimuli by changing their phenotypes to an activation state and altering the expression of inflammation-related genes. Nitric oxide (NO) is a diatomic molecule implicating in various pathological conditions including tissue damage, ER stress, obesity, and cancer. The sustained inflammatory microenvironment leads to increased NO release through the activation of the inducible nitric oxide synthase (iNOS) gene in macrophages. Here, we provide a dataset on the optimized conditions for the THP-1 differentiation and the induction of NO/iNOS signaling under inflammatory stimulus. The human monocytic cells were differentiated into adherent macrophage-like phenotype by phorbol-12-myristate-13-acetate (PMA) stimulation under optimized conditions. In this study, NO/iNOS signaling capacity and the regulation of other pro-inflammatory genes including TNF-alpha, IL-1 beta, and COX-2 in the LPS-induced THP-1 were examined. (C) 2021 Published by Elsevier Inc.