Dietary supplementation of olive leaf extract increases haematological, serum biochemical parameters and immune related genes expression level in common carp (Cyprinus carpio) juveniles

Zemheri-Navruz F., ACAR Ü., YILMAZ S.

FISH & SHELLFISH IMMUNOLOGY, vol.89, pp.672-676, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 89
  • Publication Date: 2019
  • Doi Number: 10.1016/j.fsi.2019.04.037
  • Journal Name: FISH & SHELLFISH IMMUNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.672-676
  • Keywords: Common carp, Gene expression, Olive leaf extract, Blood parameter
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

The present study investigated the effects of dietary olive leaf (Olea europea L.) extract (OLE) on some blood parameters and immune (TNF-alpha, IL-1 beta and IL-8) related genes in different tissues (head kidney, liver and spleen) and resistance of common carp, Cyprinus carpio to Edwardsiella tarda. Five diets were prepared for fed fish (mean body weight 15.90 +/- 0.93 g) with different rates of OLE (0.0%, 0.1%, 0.25%, 0.50% and 1.0%). A control diet was prepared non-supplemented with OLE. Increased haematocrit ratio, serum myeloperoxidase activity, immune response gene levels (IL-1 beta in head kidney tissue and TNF-alpha in spleen tissue) and survival rate against E. tarda particularly in the 0.1% OLE treatment group. In conclusion, results of the present study show that feeding common carp with a diet containing 1 g/kg OLE over a period of 60 days might be adequate to improve fish immune parameters, and survival rate against E. tarda. Therefore, OLE can be used as a dietary additive to prevent E. tarda in common carp.