A total of 117 taxa belonging to 42 families were collected from the Ayvacik city of Canakkale. Out of these 43 taxa were observed to be used for medicinal purposes with 54 applications. Lamiaceae dominated the list with 12 taxa followed by Asteraceae with 5 species and Malvaceae, Liliaceae, Urticaceae with 2 species each.The most commonly used taxa are Origanum majorona, O. onites, O. vulgare ssp. hirtum, Salvia fruticosa, Sideritis perfoliata, Thymus longicaulis ssp. chaubardii var. chaubardii, Lavandula stoechas ssp. stoechas. Teucrium polium, Urtica dioica, Halm sylvestris, Portulaca oleracea, Urtica rubra ssp. caucasica. Vitex agnus-castus, Vitis vinifera, Asparagus acutifolius, Foeniculum vulgare, Juniperus oxycedrus ssp. oxycedrus, and Hypericum perforation. The taxa recorded here have been mostly used for the treatment of cough (18.6%), stomachache (13.4%), kidney ailments (11.6%), cold, analgesic, diuretic and hemorrhoid (9. 3%), injuries, tonic, abdominal pain, laxative and dyspepsia (6.9%).