Poli(Rutin) micro/nanogels for biomedical applications

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Hittite Journal of Science & Engineering, vol.8, no.2, pp.179-187, 2021 (Peer-Reviewed Journal)


Flavonoids are natural phenolic compounds that are active molecules commonly found in woody and herbaceous plants and used in natural defense mechanisms of plants against harmful microorganisms. In this research, rutin (RUT) molecule which is a natural flavonoid was bounded with epoxy groups of poly(ethylene glycol) diglycidyl ether crosslinker by using micro emulsion crosslinking technique to synthesize of poly(rutin) (p(RUT)) micro/nanogels. These p(RUT) micro/nanogels showed spherical morphologic structure with 0.3 nm-2 µm dry size range due to scanning electron microscope image. Furthermore, p(RUT) micro/nanogels was found to be in injectable size range with 548±8.9 nm average size distribution in aqueous solution depends on the dynamic light scattering (DLS) measurement. In addition, the zeta potential measurements performed at different pH conditions and potentiometric titration of the prepared p(RUT) micro/nanogels was also determined and the isoelectronic point and pKa values of these micro/nanogels was estimated as pH 2.85 and 2.16, respectively. The effects of RUT and p(RUT) micro/nanogels on α-glycosidase (AG) (EC enzyme activity were investigated and it was found that RUT and p(RUT) micro/nanogels stimulate this enzyme. Moreover, fibrinogen interaction results showed that p(RUT) micro/nanogels were more compatible than RUT for vascular system with less interaction ability of fibrinogen of p(RUT) micro/nanogels. In addition, p(RUT) micro/nanogels observed highest antioxidant scavenging ability with 251.3±20.2 gallic acid equivalency total flavonoid content (TFC) at 250 µg/mL and reduced of 1.75±0.27 µmol Fe (III) even for 5 µg p(RUT) microgel/nanogels. Furthermore, no Fe (II) chelating activity was obtained for RUT monomer, but p(RUT) micro/nanogels showed significant Fe (II) chelating activity as 43.11±17.4 %.