This study investigates the surface water quality parameters of the Atikhisar Reservoir and Sari ay Creek, both of which are located in Canakkale, the north-west part of Turkey. The conductivity, COD, BOD, TP, TN, and TSS content of water in Sari ay Creek were higher than in Atikhisar Reservoir (P<0.05), however; water DO content in Sari ay Creek was less than in Atikhisar Reservoir (P<0.05). Chl-a concentration, temperature, salinity and pH of water were not statistically significant in both of the resources (P>0.05). The quality of surface water samples of Atikhisar Reservoir and Sari ay Creek were accepted first quality and third quality, respectively. The blood samples of European chub (Squalius cephalus L.) caught in the Atikhisar Reservoir (unpolluted) and Sari ay Creek (polluted) provided the data to compare the hemato-chemical effects of pollution. The WBC, MCV, MCH, Ht, Hb, GLC, TP, and GLO levels of fish in Sari ay Creek were significantly higher than those in Atikhisar Reservoir (P<0.05). The RBC, TBIL, CHOL, AST, and ALT levels of fish in Sari ay were lower than the ones in Atikhisar Reservoir (P<0.05). There were no statistically significant differences in the MCHC, TG, ALB, Ca, and P values among the fish samples of Atikhisar Reservoir and Sari ay (P>0.05). In conclusion, the data analyses infer that blood parameters can be used as an indicator of the fish health in general. On the other hand, the blood test can be used to determine fish stress due to water pollution.