In this study, the variability of airborne fungal flora and their monthly distribution in the atmosphere in 5 different locations of Canakkale Province (Turkey) were investigated by means of the petri plate gravitational method from April 1, 2000 to March 31, 2001. Samples were taken from air by exposing petri dishes with malt extract agar (MEA) and rose bengal chloramphenicol (RBCA) media for 15 min. Then growing colonies were enumerated. Fungi were isolated in 360 petri dishes used and 4105 colonies were counted. By identification of these isolations, 19 genera (Cladosporium, Alternaria, Penicillium, Phoma, Aspergillus, Botrytis, Chaetomium, Chrysosporium, Didymocladium, Doratomyces, Drechslera, Fusarium, Humicola, Mucor, Rhizoctonia, Rhizopus, Sporotricum, Trichoderma, Ulocladium), 21 species blonging to 10 genera and Mycellia sterilia were determined. The most predominants were Cladosporium (27.5%), Alternaria (18.5%), M. sterilia (13.5%), Phoma (7.9%), Penicillium (6.7%) and Aspergillus (5.9%). In addition most of fungi isolated were important aeroallergens and phythopathogens.