This study has been conducted in order to determine the impact of applied courses on the blood lipid levels of male and female students enrolled at the different departments of School of Physical Education and Sports by a comparison of blood lipid levels; having 30 male and 30 female students volunteered for the study - a total of 60 students - whose average age was 20 +/- 2,5 years. Any exercise program has not been implemented on the subjects. The applied courses in the curriculum of the freshmen year of School of Physical Education and Sports were analyzed and their impacts on the blood lipid levels of students were checked according to participation with regards to the class schedules. 5 ml blood samples taken from the forearm antecubital area of the volunteers into yellow-tap jelly tubes were analyzed by the auto analyzer in the central laboratory for their blood lipid levels such as Cholesterol, Triglyceride, LDL- Cholesterol and HDL - Cholesterol. SPSS statistical program and Mann-Whitney U test were applied in determining the difference among departments. When the findings from male subjects were analyzed, the difference in the cholesterol levels of students from Sports Management and Physical Education Teaching departments was statistically significant (p<0.05), however the variables from other departments were statistically insignificant. When findings from female subjects were analyzed, the difference among the cholesterol and HDL cholesterol levels of the students from Coaching Education and Physical Education Teaching Departments was statistically significant (p<0.05), however the variable from other departments were determined to be statistically insignificant. In conclusion, although increasing or decreasing blood lipid levels were obtained from volunteers participating in the study depending on the departments they are enrolled at, it was determined that most of the variables were within normal limits. As a result, It is thought that the decrease in cholesterol, triglyceride LDL-Cholesterol and increase HDL - Cholesterol values were depended on the applied courses in departments, and the difference between departments is depended on the course hours of departments.