''Determination of Possible Mycotoxigenic Aspergillus spp. Using Coconut Cream Agar''


Özcan G., DEMİREL ZORBA N. N.

1st International Eurasia Mycology Congress, Manisa, Turkey, 3 - 05 July 2017, vol.1, no.1, pp.215

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Manisa
  • Country: Turkey
  • Page Numbers: pp.215

Abstract

Aspergillus species are widely found in food and agricultural products.  Some of them causes disease agricultural products and some species produce mycotoxins such as aflatoxin and ochratoxin which are very important for food and agricultural products.. Bozcaada which is a province of Çanakkale is located between 390 48' northern parallel and 260 02' eastern meridians. It is located in the north-east of the Aegean Sea. Vineyards compose a large part of vegetation in Bozcaada different grape varieties are growing in Bozcaada but Karalahna and Çavuş grape varieties are unique to the island. Since Bozcaada is located at the exit of the Çanakkale Bosphorus, the northern winds are dominant in the island. Due to these winds, microflora of the island is changing continually. In this study, grape samples were collected from 2 Karalahna vineyards and 4 Çavuş vineyards in two different location of the island. A total of 11 samplings were done during two years period (2015-2016) Aspergillus isolates were obtained from DRBC and DG18 media. A total of 298 Aspergillus isolates were plated on Coconut Cream Agar for determination of mycotoxigenic species according to Dyer and McCammon’s method. Plates were incubated at 30°C for 7 days in the dark. After incubation, isolates were evaluated for colony colour, colony diameter and radiation emission at 366 nm UV wavelength. As a result, 68.9% and 60.5% of Aspergillus isolates were determined as probable mycotoxigenic species in 2015 and 2016 respectively.