Methanol extracts obtained from endemic Verbascum pseudoholotrichum Hub.- Mor., Verbascum cymigerum Hub.- Mor., Verbascum cholorostegium Bornm. & Murb., Verbascum linguifolium Hub.- Mor., Verbascum pellitum Hub.- Mor., Sideritis brevidens P. H. Davis, Sideritis cilicica Boiss. & Bal., Sideritis vuralii H. Duman & Baser, Stachys aleurites Boiss. & Heldr., and Stachys pinardii Boiss. have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608 by the disk diffusion method. Verbascum L. extracts had strong antimicrobial activity against Gram- positive bacteria and yeast cultures. The extracts of Stachys L. were effective only against bacteria. The extracts of Sideritis L. demonstrated antimicrobial effects against the bacteria and the yeast cultures used in this study.