Synthesis and characterization of poly(N-(2-mercaptoethyl) acrylamide) microgel for biomedical applications


SENGEL S. B. , ŞAHİNER N.

POLYMERS FOR ADVANCED TECHNOLOGIES, cilt.30, ss.2109-2121, 2019 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 30 Konu: 8
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/pat.4644
  • Dergi Adı: POLYMERS FOR ADVANCED TECHNOLOGIES
  • Sayfa Sayıları: ss.2109-2121

Özet

N-(2-mercaptoethyl) acrylamide (MEAM) monomer was synthesized by acrylation of cysteamine and was cross-linked with ethylene glycol dimethacrylate (EGDMA) via dispersion polymerization forming poly(N-(2-mercaptoethyl) acrylamide) (p(MEAM)) microgel. Then, the prepared microgels were tested for potential biomedical use, eg, antioxidant capacity and blood compatibility, cytotoxicity, apoptotic, and necrotic cell death; drug delivery properties were determined. Antioxidant studies of p(MEAM) microgels revealed a super antioxidant capability with total phenol content and trolox equivalent antioxidant capacity as 6.05 +/- 1.15 mg/L gallic acid equivalency and 40.96 +/- 2.40 mM trolox/g, respectively. Moreover, the blood compatibility of p(MEAM) microgels on fresh blood was resulted in lower than 1.0% hemolysis ratios for all the studied concentration range, and the blood clotting index was determined as 60.66% at 2.0 mg/mL at microgel concentration. The biocompatibility studies employing WST-1 test on L929 fibroblast cells and DLD-1 colon cancer cells have shown that p(MEAM) microgel was biocompatible up to 200 mu g/mL concentration with the cell viability values of 84.54% and 86.15% on L929 fibroblast and DLD-1 colon cancer cells, respectively. Using Captopril was used as model drug to test p(MEAM) microgel as drug delivery device for in vitro release studies at different pHs. Release profile of Captopril was found linear up to 5 hours with the released amounts of 9.81, 12.24, and 13.78 mg g(-1)microgel at the pH 1.5, 7.4, and 9.0, respectively.