Akademik Veri Yönetim Sistemi
INTERNATIONAL BALKAN AGRICULTURE CONGRESS, Çanakkale, Türkiye, 16 - 19 Kasım 2024, cilt.1, sa.1, ss.210, (Özet Bildiri)
Storage of biocontrol agents in low temperatures is a technique used to increase their availability for application periods. The purpose of this study was to determine the effect of cold storage on the longevity and parasitism performance of Trichoramma pintoi (Voegele) (Hymenoptera:Trichogrammatidae) adults in 6°C temperature for different storage periods. In the study, 3 different storage periods were used as 5, 10 and 15 days in the refrigerator. For longevity experiment, 20 female and 20 male adult parasitoids were placed into tubes and 10 females and 10 males were fed with honey, while 10 females and 10 males were unfed. They were placed into the refrigerator and checked daily to determine the longevity. Also, control was established as 10 fed and unfed females with 10 fed and unfed males. For parasitism performance experiment, tubes with adults were placed into the refrigerator and parasitoids were collected from these tubes every five days. For each storage period, 1 female and 2 male parasitoids were placed into glass tubes with 100 E. kuehniella eggs in them with 10 replicates for each storage period. Host eggs were checked daily to determine the number of parasitized eggs. These tubes were continued to be kept in the climate chamber with 24±1°C, 65-70% r.h. and 16:8 hours Light:Dark photoperiod conditions until adult emergence and the number of adults were recorded. At the end of the study, the difference between the number of parasitized eggs was statistically significant after different storage periods. The mean number of parasitized eggs in control (77,50 eggs) was higher than all of the stored eggs. The difference between the number of parasitized eggs from 5 days and 10 days of storage was not significant, while 15 days of storage was significantly lower than all other storage periods and control. Mean longevity was significantly different between fed, unfed, fed control and fed control for both females and males, which was lower in unfed control than others. There wasn’t any significant difference between males and females in unfed, fed control and unfed control, however male longevity was longer in fed adults. As the conclusion, it is possible to keep T. pintoi adults alive for two weeks in 6°C, but parasitism performance decreases as storage times increases. Adult storage can be used as a last resort to continue the life cycle of the parasitoid, but it is not suitable for long term storage of T. pintoi.
Keywords: Cold storage, Trichogramma pintoi, egg parasitoid, emergence rate