A novel impedimetric disposable immunosensor for rapid detection of a potential cancer biomarker

Asav E., Sezginturk M. K.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.66, ss.273-280, 2014 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.ijbiomac.2014.02.032
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.273-280
  • Anahtar Kelimeler: Cancer biomarkers, Biosensor, Human epidermal growth factor receptor, Electrochemical impedance spectroscopy, Single frequency impedance, GROWTH-FACTOR RECEPTOR, ACTIVATED CARBON, SENSITIVE DETECTION, SURFACE-CHEMISTRY, PROTEIN, ADSORPTION, EXPRESSION, HER-3, NANOPARTICLES, DISPERSION
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

A specific and sensitive biosensor was developed successfully for quantitative detection of human epidermal growth factor receptor by electrochemical impedance spectroscopy. Anti-human epidermal growth factor receptor antibody was covalently immobilized onto a screen-printed carbon electrode modified with a carbon nanotube. Immobilization steps were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). After human epidermal growth factor receptor ligates with anti-human epidermal growth factor receptor immobilized onto an electrode surface, charge transfer resistance changes considerably. This electrochemical response was correlated with human epidermal growth factor receptor concentration. Under optimal conditions, the proposed biosensor could detect human epidermal growth factor receptor 2 fg/mL with a linear range from 2 to 14 fg/mL, showing high sensitivity. Kramers-Kronig Transform was performed on the experimental impedance data. Meanwhile, in a biosensor system, the Single Frequency Impedance technique was first used for characterization of interaction between human epidermal growth factor receptor and anti-human epidermal growth factor receptor. Eventually, the proposed biosensor was applied to artificial serum samples spiked with human epidermal growth factor receptor. (C) 2014 Elsevier B.V. All rights reserved.