Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae)

Yücel G., Cambaz E., Çördük N.

Anadolu Tarım Bilimleri Dergisi, vol.38, no.2, pp.397-406, 2023 (Peer-Reviewed Journal)


The study aimed to determine the chromosome number and nuclear DNA content of an endemic plant, Verbascum scamandri, using 14-week-old plants germinated from seed and to determine the genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 1 mg/L 2,4-D and 9-week-old propagated plants, induced and developed on MS medium containing 2 mg/L Kin + 0.1 mg/L 2,4-D. In the mitotic chromosome counts showed that V. scamandri had 2n = 32 chromosome number. Flow cytometric analysis revealed that 14-week-old in vitro grown plants have 0.73 pg/2C ± 0.01, callus tissue has 0.76 pg/2C ± 0.02 and propagated plantlets have 0.79 pg/2C ± 0.01. The results proved that propagated plants had similar DNA content to the seed-derived plants which showed analysed plants were genetically stable.