Photoelectrocatalytic determination of NADH in a flow injection system with electropolymerized methylene blue

DİLGİN Y., GİRAY DİLGİN D., DURSUN Z., GÖKÇEL H. İ., Gligor D., Bayrak B., ...More

ELECTROCHIMICA ACTA, vol.56, no.3, pp.1138-1143, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 3
  • Publication Date: 2011
  • Doi Number: 10.1016/j.electacta.2010.10.084
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1138-1143
  • Çanakkale Onsekiz Mart University Affiliated: Yes


It was firstly described that a glassy carbon electrode electropolymerized with methylene blue shows an efficient photoelectrocatalytic activity towards NADH oxidation in a phosphate buffer solution (pH 7.0). In order to perform the photoelectrocatalytic determination of NADH in a flow injection analysis (FIA) system, a home-made flow electrochemical cell with a suitable transparent window for the irradiation of the electrode surface was constructed. The currents obtained from the photoamperometric measurements in the FIA system at optimum conditions (flow rate of carrier solution, 1.3 mL min(-1): transmission tubing length, 10 cm; injection volume, 100 mu L; and constant applied potential, +150 mV vs. Ag/AgCl) were linearly dependent on the NADH concentration and linear calibration curves were obtained in the range of 1.0 x 10(-7)-2.0 x 10(-4) M. The detection limit was found to be 4.0 x 10(-8) M for photoamperometric determination of NADH. (C) 2010 Elsevier Ltd. All rights reserved.