Akademik Veri Yönetim Sistemi
6th International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2025), Ankara, Türkiye, 25 - 27 Haziran 2025, ss.182, (Özet Bildiri)
Hypericum perforatum is a widely recognized medicinal plant known for its antimicrobial, antiinflammatory,
and antioxidant properties. Recently, its potential immunomodulatory effects have garnered
attention, particularly in experimental models such as Galleria mellonella, an invertebrate increasingly used
to investigate innate immune responses. The choice of G. mellonella in such research is especially valuable,
as its immune system shares notable similarities with that of vertebrates and supports the 3R principles by
offering an ethical, cost-effective alternative to vertebrate models. The study using this model have shown
that H. perforatum extract can influence phenoloxidase activity and the expression of antimicrobial peptides
like hemolin and gallerimycin, suggesting its ability to modulate immune signaling and effector
mechanisms. Larvae reared under controlled conditions were injected with four doses of H. perforatum
extract, and immune responses were evaluated 24 hours post-injection. Hemolymph samples were collected
to assess total hemocyte counts, phenoloxidase and antioxidant enzyme activities, as well as encapsulation
and melanization responses using standard light microscopy-based and spectroscopic techniques.
Hypericum perforatum administration modulated immune responses in G. mellonella in a dose-dependent
manner, with higher doses significantly enhancing strong encapsulation and lower doses promoting strong
melanization responses. The results indicate that H. perforatum modulates hemocyte count in a dosedependent
manner, with moderate concentrations significantly increasing total hemocyte numbers, while
the highest concentration showed no immunostimulatory effect, suggesting a potential shift toward
immunosuppression or tolerance at elevated levels. However, despite these cellular immune modulations,
Hypericum extract did not exhibit a measurable effect on DPPH radical scavenging activity in G. mellonella
hemolymph, indicating limited systemic antioxidant impact under the tested conditions. These findings
suggest that H. perforatum may act as a dose-dependent immunomodulator in G. mellonella, enhancing
cellular immune responses at moderate concentrations while showing potential immunosuppressive effects
at higher doses.