Research Information System
International Journal for Multidisciplinary Research (IJFMR), vol.4, no.6, pp.1-10, 2022 (Peer-Reviewed Journal)
The yield of fermentation process, bioethanol,
is one of the important product obtained from renewable energy sources. Yeasts
are commonly used in ethanol production due to their high ethanol tolerance,
high ethanol productivity, and their ability to ferment different kinds of
simple sugars. Saccharomyces cerevisiae
yeast species is the most commonly used in industrial ethanol production.
Therefore, isolation and identification of new yeast strains with high
fermentation capacity is very important for the bioethanol industry. The aim of
this study was isolation of novel and industrially applicable yeast species
that can be used in bioethanol production. Yeast strains were isolated from the
Dalaman, Altınbaş and Kıvırcık sarı dilim melon varieties that are unique to
Kırkağaç district of Manisa province. The fermentation ability of yeast strains
was evaluated and the selected yeast strains were identified by PCR-RFLP analysis
of 26S and ITS1-5.8S-ITS2 rDNA gene regions. Sequence analysis of the 26S
rDNA-D1/D2 gene region was used for molecular identification of yeast strains.
The phylogenetic analysis of identified yeast strains was carried out using the
MEGA XI phylogenetic analysis tool. It was determined that 14 of the 53 yeast
strains isolated were able to ferment glucose and sucrose, and four of them
were able to ferment lactose. The yeast strains having the fermentation
capacity were identified as Pichia
kluyverii, Lachancea thermotolerans, Pichia kudriavzevii, Saccharomyces cf.
cerevisiae/paradoxus and Saccharomyces
cerevisiae. These yeast strains have the potential to be used in the
bioethanol industry after other physiological and biochemical tests are
complete.