III. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 1 - 03 September 2021, pp.137
Conehead thyme (Thymbra capitata) is widely distributed on the coastlines of Mediterranean
countries and in the Aegean and Mediterranean region coastlines of our country. In addition, it
is known that it has been consumed as an insect repellent and medicinal plant since ancient
times. The most important active ingredient of T. capitata is carvacrol, and this phenolic
compound constitutes 72% of the essential oil of the plant. The antibacterial, antifungal and
antioxidant properties of this plant species are known. The great wax moth (G. mellonella) is
widely used for immune research. This invertebrate model organism is suitable for research
with human pathogens. Data from this organism are as reliable as data from mammalian models.
This study was carried out to determine the effect of antioxidant properties of T. capitataon the
antioxidant enzyme activity of the model organism Galleria mellonella hemolymph. The
areolar parts of T. capitata collected from the Çanakkale province were dried under laboratory
conditions, then the leaves were extracted and extracted with 70% ethanol in a Soxhlet device.
Stock solution was prepared by dissolving the extract in phosphate buffered saline (PBS) at a
rate of 20 mg mL-1. 18-2 mg mL-1 dilutions of this stock solution were determined as
experimental doses. According to the results of our study, T. capitata has no effect on the
amount of malondialdehyde. There was no significant difference between control and PBS
groups on superoxide dismutase and Catalase at doses below 10 mg mL-1, but it was determined
that all 10-20 mg mL-1 doses significantly reduced enzyme activities compared to control and
PBS. According to the results of our study, the use of this plant species, whose beneficial
properties are known, above a certain dose leads to negative results.