ASSESSMENT OF THE EFFECT OF THYMBRA CAPITATA EXTRACT ON GALLERIA MELLONELLA HEMOLYMPH ANTIOXIDANT ENZYMES


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Kaya S., Çetin B.

III. International Agricultural, Biological & Life Science Conference, Edirne, Türkiye, 1 - 03 Eylül 2021, ss.137

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Edirne
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.137
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Conehead thyme (Thymbra capitata) is widely distributed on the coastlines of Mediterranean

countries and in the Aegean and Mediterranean region coastlines of our country. In addition, it

is known that it has been consumed as an insect repellent and medicinal plant since ancient

times. The most important active ingredient of T. capitata is carvacrol, and this phenolic

compound constitutes 72% of the essential oil of the plant. The antibacterial, antifungal and

antioxidant properties of this plant species are known. The great wax moth (G. mellonella) is

widely used for immune research. This invertebrate model organism is suitable for research

with human pathogens. Data from this organism are as reliable as data from mammalian models.

This study was carried out to determine the effect of antioxidant properties of T. capitataon the

antioxidant enzyme activity of the model organism Galleria mellonella hemolymph. The

areolar parts of T. capitata collected from the Çanakkale province were dried under laboratory

conditions, then the leaves were extracted and extracted with 70% ethanol in a Soxhlet device.

Stock solution was prepared by dissolving the extract in phosphate buffered saline (PBS) at a

rate of 20 mg mL-1. 18-2 mg mL-1 dilutions of this stock solution were determined as

experimental doses. According to the results of our study, T. capitata has no effect on the

amount of malondialdehyde. There was no significant difference between control and PBS

groups on superoxide dismutase and Catalase at doses below 10 mg mL-1, but it was determined

that all 10-20 mg mL-1 doses significantly reduced enzyme activities compared to control and

PBS. According to the results of our study, the use of this plant species, whose beneficial

properties are known, above a certain dose leads to negative results.