Lateral flow assays: Principles, designs and labels

Bahadir E. B., Sezginturk M. K.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, cilt.82, ss.286-306, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 82
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.trac.2016.06.006
  • Dergi Adı: TRAC-TRENDS IN ANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.286-306
  • Anahtar Kelimeler: Lateral flow assays, Lateral flow devices, Immunochromatographic assays, Biosensors, Immunoassays, IMMUNOCHROMATOGRAPHIC STRIP TEST, LINKED-IMMUNOSORBENT-ASSAY, ON-SITE DETECTION, BACILLUS-ANTHRACIS SPORES, MULTICOLOR QUANTUM DOTS, ESCHERICHIA-COLI O157H7, GOLD IMMUNOASSAY STRIP, NUCLEIC-ACID BIOSENSOR, C-REACTIVE PROTEIN, RAPID DETECTION
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

Lateral flow assays (LFAs) have attracted interest due to their friendly user formats, short assay times, little interferences, low costs, and being easy by operated by non-specialized personnel. This technique is based on biochemical interaction of antigen-antibody or probe DNA-target DNA hybridization. A lateral flow assay (LFA) is composed of four parts: a sample pad, which is the area on which sample is dropped; conjugate pad, on which labeled tags combined with biorecognition elements; reaction membrane containing test line and control line for target DNA-probe DNA hybridization or antigen-antibody interaction; and absorbent pad, which reserves waste. For the construction of LFAs gold nanoparticles, colored latex beads, carbon nanoparticles, quantum dots, and enzymes are used as a label for increasing the sensitivity. In this work, the principle of LFAs, biorecognition elements, analytical performances, limits of detection (LODs), linear ranges of developed LFAs in different fields are summarized. Future perspectives in this area are also discussed. (C) 2016 Elsevier B.V. All rights reserved.