The aim of this study was to determine changes in total sialic acid (TSA), malondialdehyde (MDA), nitric oxide (NO), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) levels on sera and CSF and reduced glutathione (GSH) levels on blood in cattle with Malignant Catarrhal Fever (MCF). For this purpose 17 cattle which clinically diagnosed "head-eye form" of MCF and clinically healthy 10 cattle were evaluated. Blood and cerebrospinal fluid (CSF) were taken from the animals on the MCF diagnosed group MDA, GSH, NO, eNOS, iNOS, and TSA values were 25.65 +/- 0.42 mu mol/L, 37.21 +/- 1.12 mg/dL, 30.61 +/- 0.41 mu mol L, 4.05 +/- 0.09 U/L and 10.98 +/- 0.35 U/L, 88.33 +/- 1.03 mg/dL, on the control group 13.77 +/- 0.55 mu mol/ L, 60.06 +/- 1.73 mg/dL, 11.27 +/- 0.4 mu mol/ L, 3.12 +/- 0.18 U/L, 5.55 +/- 0.3 U/L and 63.60 +/- 1.86 mg/dL respectively, and all parameter changes between the groups were determined to be statistically significant (P< 0.001). On the CSF, no statistically significant difference between taken from MCF diagnosed group and healthy group. NO value and iNOS activity obtained from control groups CSF were relatively higher than the same group's serum whereas eNOS activities were found to be low. The study group consisting of MCF diagnosed cattle's serum were found to have NO value, eNOS and iNOS activities relatively higher than the same group's CSF values. As a result, it was concluded that there is a need for more comprehensive studies for better understanding the reason of failure to obtain the significant changes of animals diagnosed MCF that determine in blood but not in CSF.